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7/22/2014 9:46 P.M. ET

Call stands after close play at third in Nats-Rox game

DENVER -- The Nationals' attempt to keep a rally alive with a second-inning challenge came up empty Tuesday.

Bryce Harper, who drew a two-out walk, attempted to reach third from first on Ian Desmond's single up the middle. But Charlie Blackmon fielded the ball cleanly and fired to third from center field, and third baseman Nolan Arenado applied the tag ahead of a head-first-sliding Harper.

After a 2-minute, 22-second review, the umpires ruled that the call should stand, leaving the game scoreless after 1 1/2 innings.

The hit for Desmond was his sixth in as many at-bats to begin the series.

Nick Kosmider is a contributor to MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.