ATLANTA -- The Nationals challenged a close play at first base in the third inning of the Braves' 6-3 victory at Turner Field on Saturday night, and the play stood as called.

With the Braves leading, 4-1, Anthony Rendon led off with a single before Nate McLouth followed and bunted the ball toward Braves third baseman Chris Johnson, who was ruled to have thrown out McLouth at first.

Nationals manager Matt Williams then came out and challenged the call in a discussion with first-base umpire Angel Hernandez.

After the umpires reviewed the play, the call stood and the Nationals lost the ability to challenge any additional play or call in the game -- and there appeared to have been a few instances later in the game where the Nats might have been able to use that challenge. Additionally, if the call had been overturned, it would have given the Nationals runners on first and second with nobody out.

Williams wasn't pleased that the call stood.

"I'm extremely frustrated by the process at this point. If they are seeing the same feed we are seeing, I don't know how [McLouth] is out," Williams said. "So that is frustrating, because I thought he was safe. We looked at it 100 times and I believe he was safe. If that was a safe call, then we maintain our challenge."